102103013 Biotechnology Genetic Engineering & Applications Dr. Utpal Bora Web IIT Guwahati
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Role of genes within cells, genetic code, genetic elements that control gene expression
Method of creating recombinant DNA molecules
Types, biology and salient features of vectors in recombinant DNA technology: Plasmids
Types, biology and salient features of vectors in recombinant DNA technology
Safety guidelines for recombinant DNA research
Control of spills and mechanism of implementation of biosafety guidelines
Enzymes in genetic engineering: Restriction nucleases: exo & endo nucleases
Enzymes in modification- Polynucleotide phosphorylase, DNase and their mechanism of action.
Enzymes in modification- Methylases and phosphatases and their mechanism of action.
Enzymes in modification- Polynucleotide kinase, Ligases, RNase and their mechanism of action.
Methods of nucleic acid detection.
Polymerase chain reaction (PCR) and its applications
Variations in PCR and their applications
Methods of nucleic acid hybridization
Probe and target sequences
Nucleic acid mutagenesis in vivo and in vitro
Isolation and purification of nucleic acid (genomic/plasmid DNA and RNA)
Quantification and storage of nucleic acids.
Construction of cDNA library
Construction of Genomic library
Screening and preservation of DNA libraries
Gene transfer techniques: biological methods
Gene transfer techniques: chemical methods
Gene transfer techniques: physical or mechanical methods
Agro- bacterium mediated gene transfer in plants
Chloroplast transformation
Transgenic science in plant improvement
Biopharming - plants as bioreactors
Transgenic science for animal improvement
Biopharming- Animals as bioreactor for recombinant protein
Gene mapping in plants and animals
Marker-assisted selection for plant breeding and livestock improvement
Microbial biotechnology: Genetic manipulation
Engineering microbes for the production of antibiotics and enzymes
Engineering microbes for the production of insulin, growth hormones, monoclonal antibodies
Engineering microbes for clearing oil spills
Gene therapy: Introduction and Methods
Gene targeting & silencing
Gene therapy in the treatment of diseases
Challenges & future of gene therapy
M1-Problems
M2-Problems
M3-Problems
M5-Problems
M6-Problems
M7-Problems
M8-Problems
DNA Sequencing and Cloning Stategies
M4-Problems
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